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­òÛ|W3  ä  @  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ¤<?xml version="1.0"?><Features recycledIDs="5" nextValidID="9"><Feature recentID="8" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2277-3137" color="#ffffff" type="standard"/><Q name="collection_date"><V year="0" month="0"/></Q><Q name="db_xref"><V text="3133180" predef="taxon"/></Q><Q name="note"><V text="/geo_loc_name=China"/></Q><Q name="plasmid"><V text="pJNQH618-1"/></Q><Q name="collected_by"><V text="Mingju Hao"/></Q><Q name="isolation_source"><V text="urine"/></Q><Q name="lat_lon"><V text="24.26 N 118.04 E"/></Q><Q name="organism"><V text="Enterobacter intestinihominis"/></Q><Q name="host"><V text="Homo sapiens"/></Q><Q name="mol_type"><V predef="genomic DNA"/></Q><Q name="strain"><V text="JNQH618"/></Q></Feature><Feature recentID="7" name="ACN6BD_RS23585" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2277-3137" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="99708417" predef="GeneID"/></Q><Q name="old_locus_tag"><V text="ACN6BD_23580"/></Q><Q name="locus_tag"><V text="ACN6BD_RS23585"/></Q></Feature><Feature recentID="6" name="ACN6BD_RS23585" directionality="1" translationMW="31516.09" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2277-3137" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="Derived by automated computational analysis using&#10;gene prediction method: Protein Homology."/></Q><Q name="db_xref"><V text="99708417" predef="GeneID"/></Q><Q name="old_locus_tag"><V text="ACN6BD_23580"/></Q><Q name="inference"><V bool="1" text="RefSeq" predef="COORDINATES"/></Q><Q name="protein_id"><V text="WP_000027057.1"/></Q><Q name="EC_number"><V text="3.5.2.6"/></Q><Q name="product"><V text="broad-spectrum class A beta-lactamase TEM-1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="locus_tag"><V text="ACN6BD_RS23585"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="269" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment name="signal sequence" range="201-269" color="#ccffcc" type="standard" translated="1"/><Segment range="270-1061" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1232-1820" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="96-200" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-35" range="2144-2149" color="#ffffff" type="standard"/><Segment range="2150-2167" color="#ffffff" type="standard"/><Segment name="-10" range="2168-2174" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2182-2198" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  R<Notes>
<UUID>7d236638-3c2c-4b60-9517-550775a1a6eb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>&lt;html>&lt;body>Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2012.4.26</Created>
<LastModified UTC="5:45:38">2025.12.10</LastModified>
<AccessionNumber>Y14837</AccessionNumber>
<CreatedBy>GenScript</CreatedBy>
<Organism>Escherichia coli</Organism>
<SequenceClass>UNA</SequenceClass>
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<UUID>7d236638-3c2c-4b60-9517-550775a1a6eb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Description>&lt;html>&lt;body>Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2012.4.26</Created>
<LastModified UTC="5:45:1">2025.12.10</LastModified>
<AccessionNumber>Y14837</AccessionNumber>
<CreatedBy>GenScript</CreatedBy>
<Organism>Escherichia coli</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  ?     ¸  
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<UseCustomMapLabel>1</UseCustomMapLabel>
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<Created UTC="16:0:0">2012.4.26</Created>
<LastModified UTC="5:42:58">2025.12.10</LastModified>
<AccessionNumber>Y14837</AccessionNumber>
<CreatedBy>GenScript</CreatedBy>
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<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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  ¢<Notes>
<UUID>7d236638-3c2c-4b60-9517-550775a1a6eb</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pUC57</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2012.4.26</Created>
<LastModified UTC="5:42:34">2025.12.10</LastModified>
<AccessionNumber>Y14837</AccessionNumber>
<CreatedBy>GenScript</CreatedBy>
<Organism>Escherichia coli</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ­<Notes>
<UUID>23cdd778-f8b4-4c6f-91a2-803da1460f82</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pUC57-SHV-1(ecoli)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>&lt;html>&lt;body>Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.&lt;/body>&lt;/html></Description>
<Created UTC="16:0:0">2012.4.26</Created>
<LastModified UTC="5:9:49">2026.3.26</LastModified>
<AccessionNumber>Y14837</AccessionNumber>
<CreatedBy>GenScript</CreatedBy>
<Organism>Escherichia coli</Organism>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y      K         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
